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Translational Pathology & Molecular Phenotyping Core (TPMP)

Description

The overall goal of the TPMP Core is to facilitate the collection, processing, storage, and molecular analysis of biospecimens from participants enrolled on clinical and translational research studies. ICTS investigators may utilize the core to assist in the prospective collection and processing of human blood and tissue specimens associated with clinical trials, and may also search for and request previously collected biospecimens for translational and correlative research studies.

The Core also assists investigators in designing and executing studies focused on genomic and molecular (e.g. proteomic and lipidomic) analyses of study participant biospecimens. The TPMP Core is closely aligned with the Center for Biomedical Informatics (CBMI) to provide researchers with integrated access to clinical, pathology, and molecular data asssociated with each study participant biospecimen.

Services Available to ICTS Members

• Support for processing and storage of study participant blood and tissue specimens (by arrangement with study investigators on a protocol-by-protocol basis).
  
  
• Support for independent biospecimen inventory and annotation management by migrating private data sources (such as written log books and Excel spreadsheets) to a secure, enterprise-class biospecimen management system - wuTissue.
  
  
• Access to archived biospecimens (tumor tissue, non-malignant tissue, peripheral blood leukocytes, serum, plasma) and their molecular derivatives, based on current availability.
  
  
• Genomic analysis of human biospecimens using the following platforms:
  • Affymetrix GeneChip human gene expression microarrays
    - U133Plus2, Gene 1.0 ST, Exon 1.0 ST
  • Affymetrix GeneChip expression microarrays for other model organisms
    - Mouse, Rat, Yeast, Drosophila, Xenopus, etc.
  • Affymetrix GeneChip human genotyping microarrays for genotyping and copy number (aCGH) analysis
    - SNP5.0, SNP6.0
    - Targeted genotyping microarrays
  • Affymetrix GeneChip gene resequencing microarrays
  • Affymetrix GeneChip whole genome tiling microarrays for human and mouse genomes
  • ABI capillary gel electrophoresis for nucleic acid resequencing
  • ABI capillary gel electrophoresis for genotyping (SNPlex)
  • ABI real-time PCR for quantitative PCR and RT-PCR analysis
  • Directed, PCR-based gene resequencing (genomic DNA and cDNA)
  
  
• Proteomic and Lipedomic analysis of biospecimens is supported in conjunction with the Proteomics and Mass Spectrometry Core.