The ICTS Proteomics and Mass Spectrometry Program (PMSP) offers next generation mass spectrometry and data analysis tools for global and targeted protein quantification in biological fluids and tissues. Consultation on experimental design, selection of analytical platforms and sample preparation are provided. The program offers integrated proteomic workflows for discovery and verification of protein targets in biological fluids and tissues. Development of high-precision, quantitative assays is available using antibody (high-sensitivity ELISA) or antibody independent methods (stable isotope dilution MS). Phosphoproteomics of tissue samples and quantitative analysis of specific protein phosphorylation sites has recently been added as a service.
Services & Resources
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Procurement and sample preparation methods for proteomic and phosphoproteomic analyses
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Access to software tools for global (fold change) and targeted (pg/mg total protein) quantitative proteomics.
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Customized database construction for high-specificity analysis of post-translational modifications.
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Quantitative isotope-dilution mass spectrometric measurements of targeted peptides.
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Development of quantitative ELISA assays for candidate protein biomarkers using the high-sensitivity (fg/mL) Singulex platform.
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Centralized data structure for access, storage, backup and retrieval of raw and processed MS data.
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Standard and customized reports for the interpretation and publication of proteomic data sets.
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Pathways and network analysis tools that integrate RNA and protein expression data.
Barocycler
See pictures and descriptions of our barocycler setup and operation for sequential endoprotease digestion of tumor tissue.
Facilities & Other Resources
Access the standard description of PMSP facilities and other resources for grant application purposes.