1. Massively Parallel Imaging Assay Elucidates Subtle Pathogenic Phenotypes of Mitochondrial Gene Variants

Introduction: Mutations in mitochondrial genes underlie numerous neurodegenerative diseases, yet the significance of most variants is uncertain concerning disease phenotypes. Many pathogenic mutations cause visibly distinct morphological differences in afflicted cells, allowing human cells to serve as proxies for patients when studying the genetic basis of disease. This postulate provides the basis for a pooled […]

2. Translational Evidence Associating NADPH Oxidase and Heparan Sulfate Proteoglycan Activity with Cerebral Amyloid Angiopathy in Alzheimer Patient Biopsies

Introduction: Most dementia patients have co-existing vascular contributions to cognitive impairment and dementia (VCID). Cerebral amyloid angiopathy (CAA), a form of vascular dementia, bridges the interplay between VCID and Alzheimer’s Disease (AD) pathology and cooccurs in 90% of patients with AD. The hallmark pathology of CAA is characterized by amyloid-ß deposition in cerebrovasculature. Reactive oxidative […]

3. Effect of Germinal Matrix Hemorrhage-Intraventricular Hemorrhage on Neonatal Rat Brain Development: A Time Course Study

Introduction: Germinal matrix hemorrhage-intraventricular hemorrhage (GMH-IVH) is a pathologic condition which preferentially effects premature neonates, some of which later develop posthemorrhagic hydrocephalus (PHH). To date, no study has attempted to provide a head-to-head comparison of structural alterations which occur based on age of hemorrhagic insult. This study aims to provide a time course of age-dependent […]

4. Development of Sutureless Vaso-Lock for Vascular Anastomosis

Introduction: Micro- and macrovascular anastomoses involve suturing together of blood vessels, which is a critical foundational surgical skill. However, it faces many challenges, such as decade-long training and inherent dexterity of the surgeon. Procedures are long and expensive, require specialized operating rooms and equipment, making them prohibitive in many hospitals. Our team has created a […]

5. Antigen-guided Depletion of Anti-HLA Antibody-producing Cells by HLA-Fc Fusion Proteins

Introduction: Platelet transfusion and transplantation of allogeneic stem cells and solid organs are life-saving therapies. Unwanted alloantibodies to non-self human leukocyte antigens (HLA) on donor cells increase the immunological barrier to these therapies and are important causes of platelet transfusion refractoriness and graft rejection. Although the specificities of anti-HLA antibodies can be determined at the […]

6. ASOs Targeting the miR-29b Binding Site in the GRN mRNA Increase Translation of Progranulin: Potential Therapeutic Strategy for Progranulin-deficient Frontotemporal Dementia

Introduction: Frontotemporal dementia (FTD) is a devastating neurological disease with no approved treatment or cure. One common cause of FTD is heterozygous loss-of-function GRN mutations, which result in haploinsufficiency of progranulin. Antisense oligonucleotides (ASOs) are emerging as a promising therapeutic modality for neurological diseases, but ASO-based strategies for increasing target protein levels are limited. Several […]

7. Lobe Specificity of Iron Binding to Transferrin Differentially Influences the Relationship Between Bone Morphogenetic Protein 6 and Hepcidin Expression in Mice

Introduction: The mechanisms by which changes in transferrin (TF) saturation regulate hepcidin are unclear. We reported that transgenic mice expressing mutant TF that block iron binding to either N-lobe (N-bl) or C-lobe (C-bl) have differences in erythropoietin sensitivity and hepcidin regulation. To characterize the differential regulation of the gene encoding hepcidin, Hamp1, in these mice […]

8. Evolution of a Functionally Intact but Antigenically Distinct Dengue Virus Fusion Loop

Introduction: A hallmark of Dengue virus (DENV) pathogenesis is the potential for antibody dependent enhancement (ADE), which is associated with deadly DENV secondary infection, complicates the identification of correlates of protection, and negatively impacts the safety and efficacy of DENV vaccines. ADE is linked to antibodies targeting the pre-Membrane (prM) protein and the fusion loop […]

9. A Tampon-like Xerogel Device for Non-hormonal Contraception

Introduction: An unplanned pregnancy can be an emotionally devastating experience for a woman. To expand non-hormonal choices for female-controlled contraceptive methods, this research aims to develop a drug-free, tampon-like xerogel device capable of fortifying natural contraceptive mechanisms within the female reproductive tract. Methods: Xerogel devices were fabricated by lyophilization of a hydrogel comprised of Carbopol® […]